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KMID : 0358120020280010081
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Journal of the Korean Public Health Association
2002 Volume.28 No. 1 p.81 ~ p.88
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The Effect of Antioxidant Vitamins on Residual Aflatoxin B1 in Mice Serum
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Park Seon-Ja
Kim Hyoung-Kab
Chung Duck-Hwa
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Abstract
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Aflatoxin B1(AFB1) is a potent hepatotoxic and hepatocareinogenic mycotoxin in human beings. It is also well known to be accumulated in animal tissues via various metabolic pathways. This study was conducted to examine the effects of antioxidant vitamins on aflatoxin B1 accumulation in mice serum. For this work, vitamin C and vitamin E were injected with 10mg and 63,8mg per kg of body weight, respectively, through intraperitoneal(i.p.) injection to 6 week old male ICR mice. After 1 hour administration of both vitamins, 0.4mg/kg of AFB1 was injected by the same method. These administrations were repeated every 2 days over a period of 7 days(AFBi plus vitamins treated groups). On the other hand, the control group was injected only with AFB1 by the same method without vitamins. The contents of AFB1 in mice serum were determined by indirect competitive ELISA and HPLC. At the quantitative analysis of AFB1 in mice serum by ELISA, 12.28ng/ml and 18.78ng/ml of AFB1 were detected in AFB1 treated groups, but 7.60ng/ml and 4.85ng/ml of AFB1 were detected in AFB1 plus vitamin C and vitamin E treated groups, respectively. HPLC analysis of AFB1 in mice serum showed also a similar tendency as follows: 14.93, 17.33ng/ml of AFB1 in AFB1 treated group and 7.93ng/ml and 9.69ng/ml AFB1 in AFB1 plus vitamin C and vitamin E treated groups, respectively. Statistical significance was p<0.01. With regard to these data, antioxidants vitamin C and vitamin E significantly reduced AFB1 in mice serum.
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KEYWORD
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Aflatoxin B1(AFB1), antioxidant vitamin C and E, ELISA, HPLC
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